October 14, 1999 Greetings to all the Mini Analysis Program supporters! This newsletter informs the users of Mini Analysis Program about the updates and changes in the program. Mini Analysis Program version 4.3 is just released. To upgrade from v4.x to v4.3, download a patch file from our upgrade home page at http://www.syanptosoft.com. In version 4.3 users will be able to open and analyze data files with multiple input channel recordings. This feature will open up another exciting field of multiple cell recording and correlative studies. So far, researchers all over the world are actively using the program in the major fields such as miniature EPSC, EPSP recordings, amperometric recordings, calcium imaging traces, spontaneous action potential and spikes, spontaneous currents and potentials, and so on. If you have a last-minute analysis for the upcoming neuroscience meeting at Miami, please try the program. It will let you analyze your data so quickly and so completely. We also added two new domain names; www.MiniAnalysis.com and www.PeakAnalsysis.com. If you forget our domain name, just type www.MiniAnalysis.com and you will be able to reach! us at www.synaptosoft.com. Please check out the demo of Mini Analysis Program version 4.3 at http://www.synaptosoft.com now! And don't forget to send us any of your suggestions! 1. Open multiple channel file. 2. ABF Utility - Tida v2.1 file conversion. 3. Custom range fitting. 4. Horizontal slider 5. Interesting question. 6. See you all in Miami. 7. References. 1. Open multiple channel file. As more and more people are recording from multiple cells, the analysis tools for these data are in great need. The Mini Analysis Program took an initiative to head towards this direction by allowing to open multiple channel data. When you click on a data file to open, the program will prompt you with an option to display which input channel, if the data file contains more than one channel worth of data. Once the data file is open, you can easily go back and forth between different channels. This means you can detect in different channels. Each detected event will be encoded with a specific channel. You will be able to analyze these events from multiple cells in many different ways in short future (we are open to suggestions on what type of analysis to be done). 2. ABF Utility - Tida v2.1 file conversion. The latest file to be added to the ABF Utility is Tida v2.1 file type. The ABF Utility is a bundled program that allows users to convert a variety of file types to an ABF (axon binary file), so that the Mini Analysis Program will be able to open and analyze. Please inquire us about your data files. 3. Custom range fitting. In Group Analysis, now you can fit the decay of each trace or average trace within a custom range. Users can set a range by placing the two vertical line cursors to the desired position. Then the program will try to fit only within the two cursors. 4. Horizontal slider. A horizontal slider is added to the main window of the program to allow users to quickly get a sense of where in a data file the program is currently analyzing. Many users asked for this convenient feature. You can easily go to certain location in a data file by dragging this slider to desired position in time. 5. Interesting question. One user asked me recently about what will happen to the registered copies (requiring a registration key file to run) if I suddenly drop dead! Well, in the case of me disappearing from the earth, the program will be donated to the whole neuroscience community. The full version of the Mini Analysis Program, which does not require a registration key file, will be available for download at our website at http://www.synaptosoft.com soon after the tragedy happens. 6. See you all in Miami. If you would like to see the developer of the Mini Analysis Program in person, you should come to my poster. Poster - 80.12: Functional expression of AMPA receptors in nociceptive rat sensory neurons. Sunday 10/24/99, 11:00 AM LL-26 , Exhibit Hall A/B/D Session Title: Ligand-gated ion channels: glutamate receptors If you send me your poster info, I can also visit yours. 7. References Here is the list of the latest journal articles with Mini Analysis Program referenced. We welcome all your journal articles to be listed in our home page. We expect to see many more new articles to be published in 1999. When you refer to Mini Analysis program, please refer as "Mini Analysis Program (Synaptosoft Inc., www.synaptosoft.com)". If journal editors won't let you use internet address for the address of company, then just use "(Synaptosoft Inc., Leonia, NJ, USA)" as the company info. Here are recent articles from major neuroscience journals. There might be more in other journals. See our website at http://www.synaptosoft.com for complete listing. Christian Essrich, Matthias Lorez, Jack A. Benson, Jean-Marc Fritschy & Bernhard Lüscher Postsynaptic clustering of major GABAA receptor subtypes requires the 2 subunit and gephyrin Nature Neuroscience November 1998 Volume 1 Number 7 pp 563-571 Ian M. Stanford and Alison J. Cooper Presynaptic µ and Opioid Receptor Modulation of GABAA IPSCs in the Rat Globus Pallidus In Vitro J. Neurosci. 1999 19: 4796-4803 Tim Fergestad, Warren S. Davis, and Kendal Broadie The Stoned Proteins Regulate Synaptic Vesicle Recycling in the Presynaptic Terminal J. Neurosci. 1999 19: 5847-5860. S. C. Taylor, E. Carpenter, M. L. Roberts, and C. Peers Potentiation of Quantal Catecholamine Secretion by Glibenclamide: Evidence for a Novel Role of Sulphonylurea Receptors in Regulating the Ca2+ Sensitivity of Exocytosis J. Neurosci. 1999 19: 5741-5749. Daewoo Lee and Diane K. O'Dowd Fast Excitatory Synaptic Transmission Mediated by Nicotinic Acetylcholine Receptors in Drosophila Neurons J. Neurosci. 1999 19: 5311-5321. Matthew H. Higgs and Peter D. Lukasiewicz Glutamate Uptake Limits Synaptic Excitation of Retinal Ganglion Cells J. Neurosci. 1999 19: 3691-3700. Aaron DiAntonio, Sophie A. Petersen, Manfred Heckmann, and Corey S. Goodman Glutamate Receptor Expression Regulates Quantal Size and Quantal Content at the Drosophila Neuromuscular Junction J. Neurosci. 1999 19: 3023-3032. Carlos Vicario-Abejón, Carlos Collin, Ronald D. G. McKay, and Menahem Segal Neurotrophins Induce Formation of Functional Excitatory and Inhibitory Synapses between Cultured Hippocampal Neurons J. Neurosci. 1998 18: 7256-7271. K. Lee, A. K. Dixon, I. Gonzalez, E. B. Stevens, S. McNulty, R. Oles, P. J. Richardson, R. D. Pinnock, and L. Singh Bombesin-like peptides depolarize rat hippocampal interneurones through interaction with subtype 2 bombesin receptors J Physiol (Lond) 1999 518: 791-802. S. C. Taylor, M. L. Roberts, and C. Peers Acid-evoked quantal catecholamine secretion from rat phaeochromocytoma cells and its interaction with hypoxia-evoked secretion J Physiol (Lond) 1999 519: 765-774. Haruyuki Kamiya and Seiji Ozawa Dual mechanism for presynaptic modulation by axonal metabotropic glutamate receptor at the mouse mossy fibre-CA3 synapse J Physiol (Lond) 1999 518: 497-506. Paul J. Emmerson and Richard J. Miller Pre- and postsynaptic actions of opioid and orphan opioid agonists in the rat arcuate nucleus and ventromedial hypothalamus in vitro J Physiol (Lond) 1999 517: 431-445 S. C. Taylor and C. Peers Chronic hypoxia enhances the secretory response of rat phaeochromocytoma cells to acute hypoxia J Physiol (Lond) 1999 514: 483-491 Karen Zito, Dorit Parnas, Richard D. Fetter, Ehud Y. Isacoff, and Corey S. Goodman Watching a Synapse Grow: Noninvasive Confocal Imaging of Synaptic Growth in Drosophila Neuron 1999 22: 719–729. Mark N. Wu, Tim Fergestad, Thomas E. Lloyd, Yuchun He, Kendal Broadie, and Hugo J. Bellen Syntaxin 1A Interacts with Multiple Exocytic Proteins to Regulate Neurotransmitter Release In Vivo Neuron 1999 23: 593–605. Junko Yamada, Fumihito Saitow, Shin'Ichiro Satake, Toshikazu Kiyohara and Shiro Konishi GABAB receptor-mediated presynaptic inhibition of glutamatergic and GABAergic transmission in the basolateral amygdala Neuropharmacology, Volume 38, Issue 11, November 1999, Pages 1743-1753 M. Penttonen, N. Nurminen, R. Miettinen, J. Sirviö, D. A. Henze, J. Csicsvári and G. Buzsáki Ultra-slow oscillation (0.025 Hz) triggers hippocampal afterdischarges in Wistar rats Neuroscience, Volume 94, Issue 3, October 1999, Pages 735-743 Trevor J. Bushell, Chong C. Lee, Ryuichi Shigemoto and Richard J. Miller Modulation of synaptic transmission and differential localisation of mGlus in cultured hippocampal Autapses Neuropharmacology, Volume 38, Issue 10, October 1999, Pages 1553-1567 Jianguo G. Gu, Rita Bardoni, Pier Cosimo Magherini and Amy B. MacDermott Effects of the P2-purinoceptor antagonists suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid on glutamatergic synaptic transmission in rat dorsal horn neurons of the spinal cord Neuroscience Letters, Volume 253, Issue 3, 3 September 1998, Pages 167-170 If you haven't try the demo, please visit us at http://www.synaptosoft.com download the demo and try! *****Mini Analysis Program is a product of joint effort by all the users. If you have any suggestions, please let us know, we will incorporate your ideas.****** For more information, visit our website at http://www.synaptosoft.com. If you want to receive the upgrade, please download the demo at http://www.synaptosoft.com/DownloadDemo.html. If you wish to be removed from this newsletter mailing list please mailto:Reply@www.synaptosoft.com. Synaptosoft Inc. 107 Irving Street Leonia, NJ 07605 phone: 201-592-7034 fax: 201-592-7034 http://www.synaptosoft.com ftp://ftp.synaptosoft.com mailto://Webmaster@synaptosoft.com