From: Synaptosoft Subject: Fw: Date: Wednesday, November 01, 2000 9:07 AM November 1, 2000 Greetings to all the Mini Analysis Program supporters! This newsletter informs the users of Mini Analysis Program about the updates and changes in the program. The Mini Analysis Program Satelite Symposium is coming up at New Orleans in this year's Society for Neuroscience annual meeting. The complete detailed schedule is listed in this newsletter. We will see you there. Mini Analysis Program version 5.2 is just released. To upgrade from v4.x or v5.X to v5.2,download a MiniAnal.exe file from our upgrade home page at http://www.syanptosoft.com/MiniAnalysis/UpgradeOnly.html. The Most exciting features in version 5.2 includes auto-correlation and cross-correlation functions and the first modeling algorithm for the random-walk simulation. Many people have been asking about the satelite symposium at the upcoming neuroscience meeting in New Orleans. The title of the symposium is, "Analyzing Spontaneous Activities with Mini Anlaysis Program", and is scheduled for Saturday, Nov 4, at 3:00 - 5:00 PM, Morial Convention Center Room 397. No registration is required. Please attend and you will be entitled to receive a free registration of Easy Articles program (http://www.synaptosoft.com/EasyArticles/. Please check out the demo of Mini Analysis Program version 5.2 now! 1. Satelite Symposium, Saturday, Nov 4, at 3:00 - 5:00 PM, New Orleans. 2. Auto- and cross-correlation histogram 3. Random Walk modeling and curve fitting. 4. LinearRegression: r and r-squared values 5. RMS noise value to set the threshold 6. Group Episodic events in Criteria Search. 7. Re-grouping of selected trace in group analysis 8. Bugs fixed --------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 1. Satelite Symposium, Saturday, Nov 4, at 3:00 - 5:00 PM, New Orleans. Title: Analyzing Spontaneous Activities with Mini Analysis Program Location: Morial Convention Center Room: 397 Date: 11/4/00 (November 4, 2000) Time: 3:00 pm - 5:00 pm Setup: 50-75 people Please find more information in your Preliminary Program. The symposium will be divided into two parts. In the first part, Justin Lee will present the compresensive overview of Mini Analysis Program. In the second part, Dr. Jianguo Gu from Florida University will present his latest findings in spinal cord (data anlyzed with Mini Analysis Program, of course!). Justin Lee, substituting Dr. Yvonne Schmitz at Columbia University, will present the exciting results related to detection of dopamine release in cortical slices by cyclo-voltametry and amperometry, and data analysis using the newest Random Walk model. Here is the sequence of the topics to be covered in the first part of the symposium; 1 Spontaneous Activities vs. Evoked Responses 2 Study of Spontaneous Activities 3 Data Acquisition 4 Data Conversion: ABF Utility 5 Peak Detection I : Types of Peaks 6 Peak Detection II : Single Peak -Detection Parameters -Sequence of Peak Detection 7 Peak Detection III : Multiple Peaks 8 Analysis I : Grouping and Data Array 9 Analysis II : Descriptive Analysis 10 Analysis III : Group Analysis 11 Analysis IV : Statistical Analysis 12 Presentation : Graphs and Export 13 Future Directions The symposium will be held in a room for 50-75 people. We expect that there will be enough room for this first symposium. There is no registration required. Anyone who attends will be entitled for a free registration of our new program, Easy Articles. In case you cannot make it to the symposium but still would like to meet the programmer, Justin Lee, you can come to his poster on Wednesday, Nov. 8, 2:00 PM - 3:00 PM, Prog#695.2,Location C-51, Calcium permeability of kainate receptors switches off in nociceptive neurons of postnatal rats. 2. Auto- and cross-correlation histogram. Auto-correlation histogram is useful to see if there is any periodicity in the recorded activities. For example, if your cell appears to fire periodically, you can generate auto-correlation histogram of the time of occurence. The inter-event interval is calculated from each and every event to all of the other events, and all the inter-event intervals are binned and plotted as a frequency histogram. If the histogram shows a sharp peak then the cell is said to show periodicity. The function is available under Data Array menu. Cross-correlation histogram is similar to auto-correlation, except for the fact that it is for two cell recordings. The inter-event intervals from each and every event in one cell to one and every event in the other cell are binned and plotted as a frequency histogram. This plot is very useful to determine if the two cells are synaptically connected. Please ask us for the original references. 3. Random Walk modeling and curve fitting. In collaboration with Dr. David Sulzer at Columbia University, we have developed a simulation and curve fitting routine for the Random-walk model. This routine has been successfully tested for the cyclo-voltametric and amperometric detection of dopamine release from cortical slices. The program is implemented in the Group Analysis window, and it is used to estimate the dopamine release and uptake parameters by fitting a best fit curve to the actual recording. Each curve is generated from Random-walk simulation algorithm and the best fitting is achieved by the simplex fitting algorithm, which minimizes the sum of squares. The theoretical backgroud is provided at http://www.columbia.edu/~ds43/download.html and it will be discussed in more detail at the symposium. 4. LinearRegression: r and r-squared values Linear regression now adds coefficient of variations, r and r-squared values. The function is available under Data Array-> Statistical Data Analysis->Linear Regression menu. It will plot the data, display the fitted line and show the r and r-squared values. 5. RMS noise value to set the threshold. People who records and anlyzes amperometric recordings traditionally have used RMS noise as a threshold for detecting peaks. Now, Mini Analysis program will calculate the RMS noise and set the amplitude and area threshold based on this value. The function is available under File-> Measure RMS noise menu. 6. Group Episodic events in Criteria Search. If you use Mini Analysis to detect evoked responses, you will immediately notice that Mini Analysis will display traces as one continuous recording. You can reconstruct the episodic nature of the peaks by running the new function, Group Episodic Events under Events menu. Once peaks are grouped, you can go to Group Analysis window and display the grouped events as superimposed, individual or average trace. 7. Re-grouping of selected trace in group analysis. In Group Analysis window, user could click on the right mouse button on a trace and either remove the selected trace from group or export trace data. Now, you also have an option to re-group the selected trace as a group number other than the current group. This way, you can set aside the ones you originally grouped but for some reason, do not want to include in the analysis. 8. Bugs fixed Graph title causing error when it is greater than 80 characters fixed. Selecting trace by right mouse click on group analysis window fixed. Rise time is saved correctly in group analysis/amperometric data analysis/manual detection. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------- If you haven't try the demo, please visit us at http://www.synaptosoft.com, download the demo, and try! *****Mini Analysis Program is a product of joint effort by all of the users. If you have any suggestions, please let us know, we will incorporate your ideas.****** For more information, visit our website at http://www.synaptosoft.com. If you want to receive the upgrade, please download the demo at http://www.synaptosoft.com/DownloadDemo.html. If you wish to be removed from this newsletter mailing list please mailto: Reply@synaptosoft.com. Synaptosoft Inc. 107 Irving Street Leonia, NJ 07605 phone: 201-592-7034 fax: 201-592-7034 http://www.synaptosoft.com http://www.MiniAnalysis.com http://www.PeakAnalysis.com http://www.EasyArticles.com ftp://ftp.synaptosoft.com mailto:Webmaster@synaptosoft.com